Immunotherapy is a new way for treating dreadful diseases like cancer. Now-a-days, activation immunotherapy is practiced in the case of cancer treatment. Activation immunotherapy is a type of immunotherapy where the immune system is stimulated to reject and destroy cancer cells. This review mainly focuses on immunotoxins as a method of immunotherapy in treating cancer. Immunotoxins are artificially constructed by inserting a specific toxin along with an artificial antibody. Each type of cancer will have its own receptors (antigens) on its surface and antibody to that antigen is constructed artificially and a specific toxin is linked with the antibody to kill the cancer cells. This method of using immune toxins in cancer treatment is a recent approach and is still under research. The efficiency of immune toxins in killing the cancer cell lines is high but a major drawback in using these immunotoxins is that they cannot penetrate into the cancer cell mass. So special delivery mechanisms would be required in delivering these immune toxins. The method of using immunotoxins is advantageous over other techniques as they only affect the cancer cells but not the normal cells. This review mainly focuses on treatment of glioma using immunotoxins and case studies on the treatment of Glioma and the results. Also it focuses on the design, construction, delivery and efficiency of various immunotoxins in treating glioma cells. © 2015, Global Research Online. All rights reserved.