Pectinases, a cocktail enzyme consists of group of enzymes that degrade the pectin substances that is present in the middle lamella of plants. The Pectin mainly constitutes D- galacturonic acid based on a- 1, 4 glycosidic linkages and forms a macromolecule. This Pectinases enzyme breakdown the complex polysaccharides into simple molecules like galacturonic acids. As the global trend was increased to minimize the environmental pollution and utilization of natural resources. The disposal of agricultural waste from industries became a threatened problem in causing pollution. So, these wastes can be used for the production of pectinolytic enzyme as it contains enough amount of pectin that can function like support and inducer by microbes. Pectinases are most upcoming enzymes in commercial sector as it has lot of applications in food and pharmaceutical industries. This article reviews about introduction of pectinase, assay methods and important applications in different sector.