Purpose: In this study, we discuss three DTN routing protocols, these are epidemic, PRoPHET and spray and wait routing protocols. A special simulator will be used; that is opportunistic network environment (ONE) to create a network environment. Spray and wait has highest delivery rate and low latency in most of the cases. Hence, spray and wait have better performance than others. This analysis of the performance of DTN protocols helps the researcher to learn better of these protocols in the different environment. Design/methodology/approach: Delay-Tolerant Network (DTN) is a network designed to operate effectively over extreme distances, such as those encountered in space communications or on an interplanetary scale. In such an environment, nodes are occasional communication and are available among hubs, and determinations of the next node communications are not confirmed. In such network environment, the packet can be transferred by searching current efficient route available for a particular node. Due to the uncertainty of packet transfer route, DTN is affected by a variety of factors such as packet size, communication cost, node activity, etc. Findings: Spray and wait have highest delivery rate and low latency in most of the cases. Hence, spray and wait have better performance than others. Originality/value: The primary goal of the paper is to extend these works in an attempt to offer a better understanding of the behavior of different DTN routing protocols with delivery probability, latency and overhead ratio that depend on various amounts of network parameters such as buffer size, number of nodes, movement ratio, time to live, movement range, transmission range and message generation rate. In this study, we discuss three DTN routing protocols: these are epidemic, PRoPHET and spray and wait routing protocols. A special simulator will be used; that is opportunistic network environment (ONE) to create a network environment. Spray and wait have highest delivery rate and low latency in most of the cases. Hence, spray and wait have better performance than others. This analysis of the performance of DTN protocols helps the researcher to learn better of these protocols in the different environment. © 2020, Emerald Publishing Limited.