Proteins are composition of amino acid. These amino acids mainly form thousands of different proteins. P-glycoprotein (P-gp), one of the protein which is one of the plasma membrane and xenobiotic transport protein. It transports a variety of drug substrates. P-gp, which is encoded as ABCB1 and also shows a mechanism to protect the body from harmful substances. It also acts as a drug export pump. In this paper, P-gp has been taken as the target sequence and the protein is processed under homology modeling. To know the proper molecular model of the protein, the target sequence is matched with the protein structure database with the help of protein data bank to find out the template. The target sequence has been matched with protein structure database with the help of bioinformatics tool and also with some of the other programming techniques. The maximum identity accession along with the P-gp structure is identified. The homologous protein structure is now analyzed and compared with the existing P-gp structure. Copyright 2012 ACM.