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A novel approach on degradation of Benzo[a]pyrene by yeast consortium isolated from contaminated soil
Sanjeeb Mandal Kumar, A. Selvi,
Published in
2016
Volume: 8
   
Issue: 7
Pages: 80 - 93
Abstract
Benzo[a]pyrene (BaP), a high molecular weight polyaromatic hydrocarbon (HMW PAH) is commonly found incontaminated soils and considered as a potent carcinogen. Microbial degradation offers an effective approach toremove toxic pollutants. In this study, four types of yeast strains viz. Rhodotorula sp. NS01, Hanseniaspora opuntiaeNS02, Debaryomyces hansenii NS03 and Hanseniaspora valbyensis NS04 isolated from BaP contaminated soil wereused for the degradation of BaP. Identification of yeast isolates was done using 18S rRNA sequences. Out of fourconsortia studied, YC01, consisting of Rhodotorula sp. NS01, Hanseniaspora opuntiae NS02 and Debaryomyceshansenii NS03, was found to show a maximum BaP degradation of 76{\%} within 6 days in aqueous medium underoptimized conditions viz. pH 7.0, temperature 30 °C, shaking speed of 130 rpm, an inoculum dosage 3{\%} (w/v) andan initial BaP concentration of 50 mg/L. Degradation of benzo[a]pyrene by the consortium YC01 exhibited firstorder kinetics with half-life period of 3.03 days. The degradation products were monitored by Fourier transforminfrared spectroscopy (FTIR), Gas Chromatography Mass Spectrometry (GC-MS) analysis. A possible pathway ofBaP degradation by yeast consortium, YC01 was proposed. Significant role of enzymes like laccase, 1,2-dioxygenase, 2,3-dioxygenase, lignin peroxidase, manganese peroxidase and catalase were noted during the processof BaP degradation. To the best of our knowledge, this is the first report on the use of yeast consortium towards BaPdegradation.
About the journal
JournalDer Pharmacia Lettre
ISSN09755071