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A novel 'Gold on Gold' biosensing scheme for an on-fiber immunoassay
Nirmal Punjabi, J. Satija, Punjabi N, Nirmal s punjabi, Soumyo Mukherji, Satija J, , Mukherji S., Soumyo mukherji
Published in SPIE
Volume: 9506
In this paper, we propose a novel "gold on gold" biosensing scheme for absorbance based fiber-optic biosensor. First, a self-assembled monolayer of gold nanoparticles is formed at the sensing region of the fiber-optic probe by incubating an amino-silanized probe in a colloidal gold solution. Thereafter, the receptor moieties, i.e. Human immunoglobulin G (HIgG) were immobilized by using standard alkanethiol and classic carbodiimide coupling chemistry. Finally, biosensing experiments were performed with different concentrations of gold nanoparticle-tagged analyte, i.e. Goat anti- Human immunoglobulin G (Nanogold-GaHIgG). The sensor response was observed to be more than five-fold compared to the control bioassay, in which the sensor matrix was devoid of gold nanoparticle film. Also, the response was found to be ∼10 times higher compared to the FITC-tagged scheme and ∼14.5 times better compared to untagged scheme. This novel scheme also demonstrated the potential in improving the limit of detection for the fiber-optic biosensors. © 2015 SPIE.
About the journal
JournalData powered by TypesetProceedings of SPIE - The International Society for Optical Engineering
PublisherData powered by TypesetSPIE
Open AccessNo
Concepts (6)
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    Fiber-optic sensor
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    Gold nanoparticles
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    Labeled bioassay
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    Localized surface plasmon resonance
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    On-fiber immunoassay
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    Plasmonic biosensor