Keratin is the most abundant structural protein in skin, hair, wool and feathers. Keratins are proteins that form hard fibers and are components of epidermal and skeletal tissues. The aim of this study is to produce pharmaceutically and industrially important amino acids from Emu feather as source using potentially important Keratinolytic bacteria capable of producing Keratinase enzyme. Feather meal is used as sole carbon source and nitrogen source to select the isolated colonies for screening of Keratinase producing bacteria. The isolated colonies were characterized using gram staining. Five colonies were selected for producing Keratinase enzyme. Of these five colonies one showed Keratinolytic activity. The Keratinolytic activity was determined using feather as substrate. The isolated colonies were also tested for Keratinolytic activity using hair, nail and chicken feather as substrates. The optimum pH and temperature was found to be 11 and 37 °C respectively. The enzyme produced was further partially purified with acetone, ethanol and ammonium sulphate. After dialysis Keratinase is further purified with sephadex column packing in chromatography. The new isolated bacterium was used in biotechnological process involving keratin.