A total of 100 actinomycetes were isolated from the marine soil samples collected from the Ennore saltpan coastal regions of Tamil Nadu, India. All the 100 isolates were screened for their antidermatophytic secondary metabolite production by cross-streak and well-diffusion methods. Among them, 3 isolates exhibit potential antidermatophytic activity by zone of inhibition (30 mm) against the dermatophyte, Trichophyton rubrum. The isolates produce a clear zone of 30-31 mm in diameter against T. rubrum by well diffusion method. The antidermatophytic activity of the ethyl acetate extracts was 19mm (5000 μg/well) which is lesser than the zone of inhibition (35 mm) of fluconazole (1000 μg/well). The potential isolates were further studied for their morphological, physiological and biochemical characteristics. The morphological characteristics and microscopic view of all the 3 isolates suggest that they belong to the genus Streptomyces and were named as DKDVIT-1, DKDVIT-2 and DKDVIT-3. ©IJIB, All rights reserved.