Lab-on-chip technology plays a major role in developing the world. Lab-on-chip is preferred for their user-friendly approach, miniature in size and less time-consuming. One such device developed using lab-on-chip technology is the Microfluidic device used for various applications. Many conventional manufacturing techniques are involved in the fabrication of such devices. Different materials like metal, polymer are used based on the type of application. In recent days, the laser micromachining has evolved in the fabrication of microfluidic devices. A review on the fabrication of Microfluidic devices using laser processing techniques like nanosecond laser, femtosecond laser, and picoseconds laser are discussed in this paper. A detailed description is given on laser usage for rapid modeling of the lab-on-chip devices using various materials for different applications. © 2020 National Institute of Optoelectronics. All rights reserved.