A two months study was made on urinary tract infection in adults (male & female) diabetic and non-diabetic IP and OP patients. This study was basically done to identify the major causative micro-organisms (mainly bacteria) present in the urine samples collected from both catheterized and self-voided patients. During this study the following factors such as patient’s age, sex, mode of specimen collection, appearance of specimen, microscopic examination, colony count and blood sugar level were all taken into account. The study also includes the antibiotic sensitivity of various UTI bacteria. This study it is evident that E.coli is the most common clinically significant opportunistic bacteria. Klebsiella pnuemoniae was found as the second most predominant bacteria in UTI cases. This study is considered to be important because it gives us the clear picture of the common bacteria that cause UTI in diabetic/non-diabetic patients and gives us an idea about the antibiotics which are resistant and sensitive to the patients. Thus making the treatment and recovery of the patients easy for the physicians. © RJPT All right reserved.