We report an enzyme-mediated biosensor with the immobilization of silver nanoparticles (AgNPs) for the detection of organophosphorus (OP) pesticides.

Amitava Mukherjee

Centre for Nanobiotechnology

Vellore Campus

Chandra Sekaran N

Nanda Kumar D

Alex S.A

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

RSC Advances

Acetylcholinesterase (AChE)-mediated immobilization of silver nanoparticles for the detection of organophosphorus pesticides

An ultrasensitive sensor was developed for the detection of an organophosphorus compound using unmodified silver nanoparticles (AgNPs). The hydrolysis of acetylthiocholine (ATCh) by acetylcholinesterase (AChE) generates a thiol-containing compound, thiocholine, which influences the AgNP aggregation. Herein, we describe an indirect enzyme-based detection of malathion with AChE and ATCh in the presence of AgNPs using the fluorometric method. The addition of malathion to the system decreased the enzyme hydrolysis and thiocholine generation, and thus was able to quench the AgNPs, with a corresponding decrease in the emission intensity at 423nm (λex=340nm). The proposed system showed linearity in the range, 2-100fM, with an excellent limit of detection of 0.556fM by fluorescence spectroscopy. The aggregation and disaggregation of AgNPs were also supported by transmission electron microscopy and dynamic light scattering analyses. The potential application of the system was demonstrated by determination of malathion in real samples like agricultural runoff water, lake water, cabbage, and apple samples using fluorometric method, and the obtained results were further validated with HPLC. © 2015 Elsevier B.V.

Colloids and Surfaces A: Physicochemical and Engineering Aspects

Acetylcholinesterase inhibition-based ultrasensitive fluorometric detection of malathion using unmodified silver nanoparticles

A novel and highly sensitive probe for the detection of organophosphorus compounds (OPs) using acetylcholinesterase (AChE) and acetylthiocholine (ATCh) during the modulated synthesis of silver nanoparticles.

Developing acetylcholinesterase-based inhibition assay by modulated synthesis of silver nanoparticles: applications for sensing of organophosphorus pesticides

Bioprocess and Biosystems Engineering

Synthesis and characterization of cysteine functionalized silver nanoparticles for biomolecule immobilization

The Analyst

A rapid method to estimate the concentration of citrate capped silver nanoparticles from UV-visible light spectra

Journal	Data powered by TypesetRSC Advances
Publisher	Data powered by TypesetRoyal Society of Chemistry (RSC)
ISSN	20462069
Open Access	0