A highly sensitive colorimetric sensor for Hg2+ was developed with unmodified AgNPs using an enzyme-based indirect method.

Amitava Mukherjee

Centre for Nanobiotechnology

Vellore Campus

Chandra Sekaran N

Nanda Kumar D

Rajeshwari A

Alex S.A

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

New Journal of Chemistry

Due to the increasing usage of silver nanoparticles in biomedical applications, it becomes important to understand their interaction with biomolecules such as protein. In the present work, we have investigated the interaction of bovine serum albumin (BSA) with silver nanorods (SNRs) using different spectroscopic techniques. The interaction between BSA and SNRs can happen through non-covalent interactions like electrostatic and hydrophobic forces. Upon the interaction of SNRs with various concentrations of BSA, a small red-shift was found in the localized surface plasmon resonance (LSPR) peak of SNRs from UV–visible spectroscopic studies, indicating the adsorption of BSA on the surface of SNRs. The UV–visible absorption spectral studies confirmed the formation of a ground-state BSA-conjugated SNR system. The number of binding sites (n = 1.86) and binding constant (K = 8.719 × 104 L·mol− 1) were obtained to check the strength of conjugation of BSA in presence of different concentrations of SNRs from fluorescence quenching analysis. Further, DLS and zeta potential analyses proved significant binding of BSA towards the SNRs. The secondary structural changes in BSA in the presence of SNRs were obtained using circular dichroism (CD) spectroscopy and Fourier transform infrared spectroscopy (FT-IR). The unfolding behavior of BSA and BSA-conjugated SNR complex were established using the surfactant, sodium dodecyl sulfate (SDS). Finally, the influence of SNRs in the hydrolysis of BSA was investigated by assaying the esterase activity. The esterase activity of BSA was determined by the hydrolysis of p-nitrophenyl acetate (p-NPA) to 4-nitrophenol. © 2017 Elsevier B.V.

Journal of Molecular Liquids

Spectroscopic studies on the interactions of bovine serum albumin in presence of silver nanorods

In this study, we present a simple colorimetric probe for detecting chromium species, i.e. Cr(vi) and Cr(iii), in aqueous solution using as-synthesized citrate-capped silver nanoparticles without further functionalization. Two types of silver nanoparticles, AgNP-I (10 ml sodium borohydride) and AgNP-II (30 ml sodium borohydride) were synthesized by varying the volume of the reducing agent. AgNP-I was proven to be specific for Cr(iii) only whereas AgNP-II would measure total chromium in a binary mixture of Cr(iii) and Cr(vi). The probe was tested in a binary mixture at a 1:1 ratio, containing the total chromium concentrations in the range of 500-5000 ppb. The sensing method was also successfully applied in a portable colorimeter with similar binary combinations paving the way for further on-site applications. © 2014 The Royal Society of Chemistry.

Analytical Methods

Simple colorimetric sensor for Cr(iii) and Cr(vi) speciation using silver nanoparticles as a probe

ACS Sustainable Chemistry & Engineering

Label-Free Cysteamine-Capped Silver Nanoparticle-Based Colorimetric Assay for Hg(II) Detection in Water with Subnanomolar Exactitude

Particle & Particle Systems Characterization

Gold and Silver Nanomaterial-Based Optical Sensing Systems

Sensors and Actuators B: Chemical

Unmodified silver nanoparticles for rapid analysis of the organophosphorus pesticide, dipterex, often found in different waters

Talanta

Gum kondagogu reduced/stabilized silver nanoparticles as direct colorimetric sensor for the sensitive detection of Hg2+ in aqueous system

Acetylcholinesterase inhibition-based colorimetric determination of Hg2+ using unmodified silver nanoparticles

Journal	Data powered by TypesetNew Journal of Chemistry
Publisher	Data powered by TypesetRoyal Society of Chemistry (RSC)
ISSN	1144-0546
Open Access	0