Classical synthesis of mesoporous material MCM-41 with the hexagonal arrangement of pores was obtained using n-cetyltrimethylammoniumbromide (CTAB) and Tetraethoxysilane (TEOS). The surface characteristics, pore structure and thermal degradation of as synthesized, calcined at 250 °C and 550 °C MCM-41 were characterized based on the analyses of the nitrogen adsorption/desorption isotherms, powder X-ray diffraction (XRD) patterns and thermo gravimetric analysis (TGA). The adsorption of organic dye (methylene blue) was studied to examine the potential of MCM-41 for the removal of dyes from aqueous solution. The adsorption of methylene blue on MCM-41 with respect to contact time, pH, and temperature was measured to provide more information about the adsorption characteristics of MCM-41. Both Langmuir and Freundlich adsorption models were applied to describe the equilibrium isotherms. The kinetic studies show that the adsorption of dye on MCM-41 follows the second-order kinetics.