We have implemented an all-fiber optical delay line using two linearly chirped fiber Bragg gratings cascaded in reverse order and all-fiber optics components. The features of the proposed all-fiber based technique for variable delay line are discussed theoretically and demonstrated experimentally. The non-invasive cross-sectional images of biomedical samples as well as a transparent glass plate obtained with implemented all-fiber delay line having the axial resolution of 100 μm and the dynamic range of 50dB are presented to validates the imaging performance and demonstrate the feasibility of the delay line for optical coherence tomography. © 2005 Optical Society of America.