The prime purpose of this research is to evaluate the influence of foam separation, an adsorptive bubble separation technique, employed as a finer treatment technique after biosorption. Cetyl Trimethyl Ammonium Bromide (CTAB) played the multiple roles of foam production, phenol elimination, and microbial collection and hence acts as a percentage promoter. The process has its own merits of lower capital investment, less operating and maintenance cost, less power consumption, relatively faster ad eco friendly technique. The effect of several working parameters, such as initial feed concentration, equilibrium time, biosorbent dose, pH, liquid pool height, surfactant concentration, and air flow rate, were experimentally explored. The higher floatability of Saccharomyces cerevisiae was obtained in a maximum time of 10 min. Among the models tested, Fowler- Guggenheim model (R2=0.9703) was the most adequate. The experimental data fitted pseudo second order kinetics with a kinetic constant of 5.5425X105hr1. Fourier transform infrared spectroscopy (FTIR) spectroscopy revealed the active participation of imidazole in the biosorptive foam separation process. Electro kinetic measurements were carried out to determine the iso electric point (IEP) of the Saccharomyces cerevisiae cells. The zeta potential profile of the baker's yeast cell was affected by the presence of phenol at different pH values. The recovery of phenol loaded biomass and final traces of phenol by flotation were found to be 99.75%. © 2019 American Institute of Chemical Engineers. All rights reserved.