Alcohol blended with gasoline is generally termed as Gasohol. Gasohol fuel will reduce Carbon monoxide (CO), Nitrous oxide (NOx), Hydro carbon (HC) emissions and it will increase Carbon dioxide (CO2). The regulated and unregulated emissions raise with increase in the Ethanol percentage of the fuel. For the reduction of both regulated and unregulated emissions new catalyst (Vanadium Pentoxide/Cobalt oxide) were coated over the metal substrate of the conventional catalytic converter. The performance of Vanadium Pentoxide/Cobalt oxide coated Three-way catalytic converter (TWC) and emissions test on a single cylinder four stroke petrol engine was conducted on the both regulated and unregulated emissions. The recorded emissions results were compared with conventional and Vanadium Pentoxide/Cobalt oxide coated catalytic converter. The experimental results suggest that Vanadium Pentoxide/Cobalt oxide coated catalytic converter reduces 55%, 38% and 45% of HC, CO and NOx respectively. The reduction in unregulated emissions like acetaldehyde, acrolein, propionaldehyde and paraldehyde was reduced by 51%, 48%, 44% and 70% due to Three-way catalytic converter respectively. © 2020 Lavoisier. All rights reserved.