Microbes are omnipresent in nature with different forms of shape, size, colony morphology and substrate interaction modes. Microbial biofilm is a unique assemblage of microbes on the surface of the substrate. Recently it has fascinated the scientific community owing to its special properties which makes it notorious as well as a worthy living system. The establishment of the biofilm ecosystem is a multi step process which involves quorum sensing communication mechanism. The interaction of the biofilm with its immediate environment, its applications and some of the strategies to remove the notorious biofilm have been described in this article.