ABSTRACT
The development of microbial enzymes for commercial use is a specialized field which requires. Meticulous screening of new and improved strains from potential sources and Scaling up of enzyme production by optimizing conditions of fermentation. Xylanase is the name given to a class of enzymes which degrade the linear polysaccharide beta-1,4-xylan into xylose. These days, xylanase are the enzymes on demand in pulp and paper industry Enzyme application improves pulp fibrillation and water retention, and selective removal of xylan from dissolving pulps. Three-quarters of the market is for enzymes involved in the hydrolysis of natural polymers, microbial exploitation with regard to xylanase production is a spectacular advancement with scientific progress in commercial scale of manufacturing of paper. Microorganisms are rich sources of xylanase enzymes. In our research study we have screened Actinomycetes which produce extra-cellular xylanase. The structural diversity associated with the xylan component from different plants gives us concept for requirement of various xylan-degrading enzymes which can utilize different lignocellulosic substrates those are being used as raw materials for the paper industry. In order to use xylanase for pulp treatment, it is required that enzymes should not have any additional cellulolytic activity, since the quality of paper will be adversely affected by cellulase. To overcome this problem, screening and tentative optimization of microbial xylanase was carried out by collecting soil samples from Chittoor paper industry waste.
Key Words: Xylanase, Lignocellulosic substrate, Paper industry, Cellulosic activity