The estimation of Hg2+ in a buffer system (Tris buffer; pH 7.4) has been performed using a novel, enzyme-based, indirect detection method with the aid of non-functionalized AgNPs.

Amitava Mukherjee

Centre for Nanobiotechnology

Vellore Campus

Chandra Sekaran N

Kumar D.N

Rajeshwari A

Alex S.A

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Analytical Methods

An efficient detection method for Hg (II) ions at physiological pH (pH7.4) was developed using tween 20-modified gold nanorods (NRs) in the presence of dithiothreitol (DTT). Thiol groups (-SH) at the end of DTT have a higher affinity towards gold atoms, and they can covalently interact with gold NRs and leads to their aggregation. The addition of Hg(II) ions prevents the aggregation of gold NRs due to the covalent bond formation between the -SH group of DTT and Hg(II) ions in the buffer system. The changes in the longitudinal surface plasmon resonance peak of gold NRs were characterized using a UV-visible spectrophotometer. The absorption intensity peak of gold NRs at 679nm was observed to reduce after interaction with DTT, and the absorption intensity was noted to increase by increasing the concentration of Hg(II) ions. The TEM analysis confirms the morphological changes of gold NRs before and after addition of Hg(II) ions in the presence of DTT. Further, the aggregation and disaggregation of gold NRs were confirmed by particle size and zeta potential analysis. The developed method shows an excellent linearity (y=0.001x+0.794) for the graph plotted between the absorption ratio and Hg(II) concentration (1 to 100pM) under the optimized conditions. The limit of detection was noted to be 0.42pM in the buffer system. The developed method was tested in simulated body fluid, and it was found to have a good recovery rate.

Materials Science and Engineering: C

Anti-aggregation-based spectrometric detection of Hg(II) at physiological pH using gold nanorods

A method for the sensitive and selective determination of Hg2+ was developed based on the aggregation and re-aggregation of silver nanorods (SNRs) in the presence of dithiothreitol.

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Conclusion

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Journal	Data powered by TypesetAnalytical Methods
Publisher	Data powered by TypesetRoyal Society of Chemistry (RSC)
ISSN	1759-9660
Open Access	0