Optic fibre plays a major role in the access part of the network. Hybrid Passive Optical Network combines the advantages of TDM based EPON Network and the WDM PON network. The objective of this paper is to compare different modulation formats in a 16-channel Hybrid Passive Optical Network and to analyse their performance. The network has a transmission rate of 10 Gbps per channel. Different modulation formats like Non return to zero (NRZ), Return to Zero (RZ), Carrier Suppressed RZ (CSRZ), Duobinary (DB) and Modified Duobinary formats are compared and their performances are evaluated. Simulation results are obtained using Optisystem. It is seen that DB Modulation format provides a longer reach equal to 105km and Mod DB formats is the second best providing a maximum reach of up to 75 km. © 2006-2018 Asian Research Publishing Network (ARPN).