Our continuous searches for antagonistic actinobacteria lead to the isolation of Streptomyces VITDDK1 spp.from the soil sample collected at the Ennore saltpan region, Tamil Nadu, India. The isolate exhibited significant antibacterial activity against the gram negative pathogenic bacteria, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa and antifungal activity against pathogenic Aspergillus flavus. Also the isolate possessed fairly moderate chitinolytic activity. Morphological and chemotaxonomic studies showed that the isolate belonged to the genus Streptomyces. Based on polyphasic taxonomy and phylogeny the isolate was designated as Streptomyces VITDDK1 spp. Further the isolate showed 99% similarity with the strain Streptomyces sp. J-1.