The study was done to evaluate the anthelmintic activity of rhizome extract of Acorus calamus in comparison with commercial beta and alpha asarone against earthworms. Different concentrations of asarone (1-5 mg/mL) and rhizome extract (2-10 mg/mL) were studied. The evaluation parameters involved the determination of time of paralysis and time of death of the worms. Piperazine citrate and alpendazole was used as standard at 10 mg/mL concentration and saline as control. The results were dose dependent and higher activity was observed in beta asarone at 5 mg/mL concentration compared to alpha asarone and rhizome extract.