Andaman and Nicobar Islands, considered as one of the important marine ecosystem in India and being considered as the richest source for microbial community. The marine microbial taxa and actinomycetes diversity are not been fully explored. Actinomycetes were isolated from marine soil samples and screened for antibacterial activity. Out of eight isolates, the isolate AN1 isolated from soil sample collected from Elephanta beach showed significant antibacterial activity against ATCC strains Staphylococcus aureus (19 mm), Bacillus cereus (17 mm) and Salmonella paratyphi A (16.5 mm). The ethyl acetate (EA) extract of the isolate showed increased activity against Staphylococcus aureus (22.1 mm), Bacillus cereus (19.5 mm) and Salmonella paratyphi A (19.6 mm). The standard antibiotic streptomycin disc (25μg) was used as positive control. The isolate AN1 were subjected to biochemical characterization and identified as Streptomyces species. The result of this study suggests that the Streptomyces species could be a promising source for potent antibacterial agents.