Marine Streptomyces sp.VITBRK3 isolated from the sediment sample collectedat the Marakkanamcoast located in the Southeast coastal region of Bay of Bengal, India. The ethyl acetate extract was prepared from the culture supernatant of Streptomyces sp.VITBRK3.The anti-MRSA compound was purified by silica gel column chromatography and HPLC. The purified compound was identified by using 1H and 13C NMR spectral data. The anti-MRSA compound was compared with the reference compounds available in the mass spectra library of National Institute for Standards and Technology (NIST).The anti-MRSA activity was carried out according to the National Committee for Clinical Laboratory Standards (NCCLS) guidelines. Analysis of spectral data resulted in identification of anti-MRSA compound as 2,4-dichloro-5-sulfamoyl benzoic acid. The anti-MRSA activity of the purified compound showed the MIC50 value ranged from 0.8to 4.00 μg/mL against the Staphylococcal strains. The results of the study suggest that Streptomyces sp.VITBRK3 is the potential source for the anti-MRSA compound.