The green synthesis of the metal silver (Ag) nanoparticles was successfully performed by one step procedure of using reducing agents of M. pruriens seed extract. The rod-shaped of size 20-60 nm Ag nanoparticles was used in the medical application of lung cancer under optimized conditions. The characterization of the biosynthesized nanoparticles was done using UV-Vis spectroscopy, Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and Energy dispersion analysis of X-ray spectroscopy (EDAX). The FTIR analysis confirms the occurrence of functional groups which proved to be responsible for the stabilization or reduction of these nanoparticles. The Ag nanoparticles in dose-dependent manner was persistent in inhibiting the lung cancer cell line A549 growth which was evaluated using the MTT assay which was evaluated with the IC50 value of 50µg when compared with the standard commercialized drug. © RJPT All right reserved.