Discovery of Bioactive compounds from marine sources especially fishes have always been of great importance since several decades and it is much more essential to identify novel natural products which might possess significant properties to treat various diseases. The main objective of this study is to evaluate the in vitro antibacterial, antifungal and larvicidal activities of various crude tissue extracts of the puffer fish Takifugu oblongus collected from the South -East Coast of India. The amount of protein present in each tissue was determined by spectrophotometric methods. The in vitro antibacterial and antifungal activities of the tissue extracts against various bacterial and fungal pathogens were investigated by well diffusion method. Larvicidal activity of the tissue extracts was assessed by analyzing the mortality rate of all tested larvae. Muscle extract showed highest protein content (340 µg/ml), whilst gonads extract showed least protein concentration (94 µg/ml) in the puffer fish. The measured zones of inhibition of various pathogens by different tissue extracts proved that gonads extract possessed highly commendable antibacterial activity against most bacterial strains. And muscle extract recorded lowest antibacterial activity. No antifungal activity was observed with all the tissue extracts. Though all the tissue extracts produced significant larvicidal activities, maximum activity was noticed with skin extract, whereas muscle extract showed the least. The crude tissue extracts of the puffer fish Takifugu oblongus showed noteworthy bioactivities and so further studies may be recommended to identify the natural bioactive compounds, so that they could be promising candidates for drug development. © 2016, International Journal of Toxicological and Pharmacological Research. All rights reserved.