Copper-chitosan nanocomposite (Cu-CS NC) was prepared by incorporating copper onto chitosan using ethylene glycol by microwave assisted method. Ethylene glycol was used as a solvent as well as a reductant for preparing thenanocomposites. The antibacterial activity was tested against Bacillus cereus, Staphylococcus aureus, Escherichia coli and Proteus mirabilis by Mueller-Hinton agar disk-diffusion method. The observed activity was found to be superior when compared to bare Cu nanoparticles for most of the bacteria.