Background: The silver nanoparticles (AgNPs) have a significant range of application in the field of biomedical technology. This can be subjected in agriculture by biological synthesis method using microbes which may inhibit disease-causing pathogens in agriculture, typically it confused on management of pathogens. Materials and Method: In this present investigation, Rhizobium sp. was isolated from cultivable soil sample for the synthesis of AgNPs. The synthesized silver nanoparticles were characterized using UV-Visible spectroscopy, Scanning electron microscopy shows regular circular shape of AgNPs followed by energy dispersive X-ray (EDX) spectrometers confirms the presences of elemental composition of materials. Transmission electron microscopy was done for the structural analysis and the surface study of synthesized AgNPs was studies by Atomic forced microscopy. Results: The surface plasmon resonances at 425 nm confirm the AgNPs formation and morphology of the nanoparticles are polydispersed with 25–70 nm in size confirmed by the microscopic techniques. The synthesized silver nanoparticles were examined against disease-causing fungal pathogens such as Aspergillus niger, Candida albicans, and Aspergillus fumigatus in which Candida Albicans showed a maximum zone of inhibition of 15 mm at 75 µL of AgNPs. Conclusion: Finally, the silver nanoparticles were used to perform free radicals scavenging activity. © 2019, Indian Journal of Public Health Research and Development. All rights reserved.