Kerala is endowed with rich biodiversity of medicinal plants which are widely used in various traditional medicinal systems like Ayurveda which are potential sources of bioactive constituents with effective and safer drugs. The present study is to evaluate the antibacterial, antioxidant and antiproliferative activity of traditional medicinal plants Biophytum sensitivum (L.) DC, Curculiogo orchioides Gaertn. and Cynodon dactylon (L.) Pers. The petroleum ether extract of Curculiogo orchioides showed significant antibacterial activity against pathogenic strains of bacteria. Among the other extracts methanolic extract of Curculiogo orchioides showed higher phenolic and flavanoid content and significant DPPH scavenging and reducing power activity. The antiproliferative activity was tested against Hep G2 (hepatocellular liver carcinoma) in which methanolic extract of Biophytum sensitivum and Curculiogo orchioides showed strong anticancer activity with an IC50 108.72 and 127.12μg/ml respectively. Our study confirmed the ethanobotanical and traditional medicinal usage of the three medicinal plants Biophytum sensitivum, Curculiogo orchioides and Cynodon dactylon.