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Antioxidant RichMorus albaleaf Extract Induces Apoptosis in Human Colon and Breast Cancer Cells by the Downregulation of Nitric Oxide Produced by Inducible Nitric Oxide Synthase
Sureshkumar T, Satheeshkumar P.K, Priya S.
Published in Informa UK Limited
2013
Volume: 65
   
Issue: 2
Pages: 305 - 310
Abstract
Morus species had been used widely in the traditional medicines for various diseases. In this study we report the in vitro antiproliferative activity of the methanol extract of Morus alba. The extract is capable of inducing cytotoxicity in human colon cancer (HCT-15) cells (IC(50) = 13.8 μg/ml) and breast cancer (MCF-7) cells (IC(50) = 9.2 μg/ml), resulted in significant morphological changes of the cells, fragmentation of DNA, and caspase-3 activation- characteristics of apoptosis. It downregulated the amount of nitric oxide (NO) produced as a result of inducible nitric oxide (iNOS) activation. The HPLC analysis of the extract showed epicatechin (20%), myricetin (10%), quercetin hydrate (12%), luteolin (12%), and kaempferol (6%) as the major active components and ascorbic acid, gallic acid, pelargonidine, and p-coumaric acid as the minor components. To the best of our knowledge, this is the first report showing the downregulation of iNOS and induction of apoptosis by M. alba extract.
About the journal
JournalNutrition and Cancer
PublisherInforma UK Limited
ISSN0163-5581
Open Access0