Antiviral activity of dasyscyphin C (C28H40O8) extracted from leaves of Eclipta prostrata was evaluated against fish nodavirus, grouper nervous necrosis virus (GNNV) infected SIGE (Sahul Indian Grouper Eye) cell lines under in vitro conditions. Nodavirus infection to SIGE cells was confirmed by cytopathic effect (CPE) and reverse transcriptase-polymerase chain reaction. The susceptibility of the virus to the isolated compound was confirmed by measuring the virus titre (TCID50/ml) at every 24 hrs. The isolated compound was found to be effective in inhibiting the proliferation of fish nodavirus (FNV) infected in the SIGE cell lines at 20μg/mL and the cell viability was decreased to 22% as determined by MTT assay. The virus titre (TCID50/ml) was 3.7 at the end of 7th day in dasyscyphin C treated SIGE cells post inoculated with nodavirus. Based on the results it can be concluded that dasyscyphin C may be used as antiviral agent against fish nodavirus. © 2010 Krishnan K, et al.

Krishnan V

Centre for Bio-Separation and Technology

Vellore Campus

Kannabiran K

Department of Bio-Medical Sciences

School of Bio Sciences and Technology

Khanna V.G

Hameed S.

Fulltext

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Journal of Antivirals & Antiretrovirals

Viral nervous necrosis (VNN) has emerged to become a major problem in the culture of larval and juvenile marine fish worldwide. Bioactive phytochemicals isolated from commonly available medicinal plants are often screened for their efficacy in controlling fish viral diseases. Occurrence of newer viral strains and resistance to existing antiviral drugs are problems currently associated with treatment of VNN, which necessitates looking for alternate sources for effective antiviral drugs. The aim of the present study was to screen antiviral potential of gymnemagenol (C30H50O4) previously extracted from leaves of Gymnema sylvestre. The fish nodavirus, grouper nervous necrosis virus (GNNV) in infected Sahul Indian Grouper Eye (SIGE) cell lines were used to study the antiviral activity of gymnemagenol under in vitro conditions. The susceptibility of the virus to gymnemagenol was confirmed by measuring the viral titre (TCID50 mL-1) in virus-infected SIGE cells every 24 h. Gymnemagenol at 20 μg mL-1 inhibited the proliferation of GNNV to 53% at the end of the 6th d by inhibiting the proliferation of GNNV-infected SIGE cells. The viable SIGE cells were reduced to 47% as determined by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The viral titre (TCID50 mL-1) was also reduced to log 2.8 at the end of the 7th d in gymnemagenol-treated SIGE cells after inoculated with GNNV when compared to untreated control SIGE cell viral titre (log 4.1). Based on our results it can be concluded that gymnemagenol could be used as an antiviral agent against GNNV infection. © 2011 Science Press, Ocean University of China and Springer-Verlag Berlin Heidelberg.

Journal of Ocean University of China

Inhibition of fish nodavirus by gymnemagenol extracted from Gymnema sylvestre

Antiviral Activity of Dasyscyphin C Extracted from Eclipta Prostrata Against Fish Nodavirus

Journal	Journal of Antivirals & Antiretrovirals
Publisher	OMICS Publishing Group
ISSN	19485964
Open Access	0