The constantly rising number of limb stroke survivors and amputees has motivated the development of intelligent prosthetic/rehabilitation devices for their arm function restoration. The device often integrates a pattern recognition (PR) algorithm that decodes amputees’ limb movement intent from electromyogram (EMG) signals, characterized by neural information and symmetric distribution. However, the control performance of the prostheses mostly rely on the interrelations among multiple dynamic factors of feature set, windowing parameters, and signal conditioning that have rarely been jointly investigated to date. This study systematically investigated the interaction effects of these dynamic factors on the performance of EMG-PR system towards constructing optimal parameters for accurately robust movement intent decoding in the context of prosthetic control. In this regard, the interaction effects of various features across window lengths (50 ms~300 ms), increments (50 ms~125 ms), robustness to external interferences and sensor channels (2 ch~6 ch), were examined using EMG signals obtained from twelve subjects through a symmetrical movement elicitation protocol. Compared to single features, multiple features consistently achieved minimum decoding error below 10% across optimal windowing parameters of 250 ms/100 ms. Also, the multiple features showed high robustness to additive noise with obvious trade-offs between accuracy and computation time. Consequently, our findings may provide proper insight for appropriate parameter selection in the context of robust PR-based control strategy for intelligent rehabilitation device. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.