Actinomycetes are a group of Gram positive bacteria with high G+C Content. The presence and distribution of actinomycetes have been demonstrated to be associated with their various ecological habitats, including beach sand and sea water. Marine actinomycetes are capable of producing different types of novel secondary metabolites. Many of these metabolites possess biological activities and have the potential to be developed as therapeutic agents. Marine actinomycetes are still a prolific but underexploited source for the discovery of novel secondary metabolites. The aim of the present study was to evaluate the cytotoxic and genotoxic effect of a compound previously extracted from marine Streptomyces sp. The strain was isolated from sediment samples collected at the Marakkanam coast of Bay of Bengal, India. Cytotoxicity of the extracted compound was assessed on MDA-MB-435S (Breast cancer cell line) and WRL- 68 (Liver cell line). Genotoxicity was tested by assay of chromosomal aberrations and micronucleus test. The compound was found to be more toxic to MDA-MB-435S cell line when compared to WRL-68 cell line. The compound showed the Ic50 value of 65μg/ml against MDA-MB-435S cells and against WRL-68 cell line it showed the Ic50 value of 250 μg/ml. The exposure of compound on chromosomes did not show any aberrations, gaps and chromatid breaks. Based on the results it can be concluded that the extracted compound possess less cytotoxicity and genotoxicity on normal cells.