The objective of this study was to screen pectin lyase producing Bacillus from coconut waste. Soil enzymes are the biocatalysts which mediate all the synthetic and degradative reactions in living organisms. They increase the rate of slow and imperceptible reactions without undergoing any net change and are remarkable because of their extraordinary specificity and catalytic power. However, in many cases the enzyme activities can still act on the same composition, as the composition can have a complex chemical structure having various types of chemical bonds, requiring different enzyme activities for breakdown. Such pectinase composition often contains one or more of the following activities: polygalacturonase, pectin lyase and pectin methyl esterase. Pectinase preparations are often used in fruit juice processing. Pectin lyase that hydrolyze the pectic substances, present mostly in plants. Although they can be derived from several sources, such as plants, animals and micro-organisms, the enzymes from microbial sources generally meet industrial demands.