Objective: Paper currency notes are found to contaminate with microbes and the dissemination of antibiotic resistance is a global health problem. The aim of this study was to evaluate the prevalence of multi-drug resistant bacteria in the Indian currency notes. Study design: For this study, the Indian currency notes (rupee notes 10, 20 and 50) were collected from Tamil Nadu, Karnataka, West Bengal, Assam, New Delhi and Gujarat using random sampling technique. The sampling was categorised into; category 1: local markets, beggars, street vendors, conductors, hospitals and Category 2: supermarkets, banks including ATM machines, and hotels. Method: Spread plate technique, specific media and biochemical tests were used for the bacterial isolation and identification. Identification of multiple drug resistance, PCR-based screening of resistance genes and conjugation studies were performed. Results: The total of 75 bacteria was isolated including Bacillus sp., Streptococcus sp., Staphylococcus sp., Staphylococcus aureus, Escherichia coli, Klebsiella sp., Pseudomonas sp. and Salmonella sp. Multi-drug resistance was observed in 32/75 (43%) isolates and the prevalence of 25% ertapenem-resistant and 21% cefotaxime resistant bacteria was observed. The presence of blaCTX-M-9 in three isolates such as E. coli (two) and Klebsiella sp. was screened and E. coli isolates were found to involve in conjugating its resistance to E. coli AB1157. Conclusions: Our study showed the prevalence of multiple drug resistant bacteria in the Indian currency notes and also in disseminating its resistance. © 2018, Indian Journal of Public Health Research and Development. All rights reserved.