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Bio-mimetic mineralization potential of collagen hydrolysate obtained from chromium tanned leather waste
Banerjee P, , Chandra Babu N.K,
Published in Elsevier BV
2015
PMID: 25686958
Volume: 49
   
Pages: 338 - 347
Abstract
Hydroxyapatite (HA) ceramics serve as an alternative to autogenous-free bone grafting by virtue of their excellent biocompatibility. However, chemically synthesized HA lacks the strong load-bearing capacity as required by bone. The bio-mimetic growth of HA crystals on collagen surface provides a feasible solution for synthesizing bone substitutes with the desired properties. This study deals with the utilization of the collagen hydrolysate recovered from leather waste as a substrate for promoting HA crystal growth. Bio-mimetic growth of HA was induced by subjecting the hydrolysate to various mineralization conditions. Parameters that would have a direct effect on crystal growth were varied to determine the optimal conditions necessary. Maximum mineralization was achieved with a combination of 10 mM of CaCl2, 5 mM of Na2HPO4, 100 mM of NaCl and 0.575% glutaraldehyde at a pH of 7.4. The metal-protein interactions leading to formation of HA were identified through Fourier-transform infrared (FTIR) spectroscopy and x-ray diffraction (XRD) studies. The crystal dimensions were determined to be in the nanoscale range by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The size and crystallinity of bio-mimetically grown HA indicate that hydrolysate from leather waste can be used as an ideal alternative substrate for bone growth. © 2015 Elsevier B.V.
About the journal
JournalData powered by TypesetMaterials Science and Engineering: C
PublisherData powered by TypesetElsevier BV
ISSN0928-4931
Open Access0