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Bioactivity of Pyrrolo[1,2-A]pyrazine-1,4-dione,hexahydro-3-(phenylmethyl)- Extracted from Streptomyces sp. VITPK9 Isolated from the Salt Spring Habitat of Manipur, India
P. Sanjenbam,
Published in Medknow Publications
2016
Volume: 10
   
Issue: 4
Pages: 265 - 270
Abstract
Aim: The aim of the present study was to evaluate the biological activity of pyrrolo[1,2-A]pyrazine-1,4-dione,hexahydro-3-(phenylmethyl)- (PPDHP) extracted from Streptomyces sp. VITPK9, isolated from the brine spring of Thoubal district, Manipur, India. Materials and Methods: Hemolytic effect of the lead compound was determined, and human erythrocytes (red blood cells) were collected and used within 24 h. For genotoxicity study, chromosome preparations were obtained from phytohemagglutinin-stimulated peripheral blood lymphocytes. The viability of cells was evaluated by 3-(4,5-dimethythiazol-.2-yl)-2,5-diphenyl tetrazolium bromide assay on RAW 264.7 cell lines. Results and Discussion: The antifungal compound PPDHP showed mild hemolytic activity with effective concentration 50% value of 115.5 μg/mL on human erythrocytes. Cytotoxicity study on normal cell-line RAW 264 exhibited a moderate toxicity with 50% inhibitory concentration value of 500 μg/mL. Genotoxicity study revealed that the compound showed minimal chromosomal aberrations when compared to streptomycin. Conclusion: The results of the study suggest that the diketopiperazine type of compounds extracted from Streptomyces sp. VITPK9 can be used as the potential antifungal compound.
About the journal
JournalAsian Journal of Pharmaceutics
PublisherMedknow Publications
ISSN09738398