Bioanalytical method for the measurement of rabeprazole in human plasma was developed and validated. Rabeprazole was resolved on a reverse phase C18 column from the endogenous plasma components by isocratic elution of 30% 25mM phosphate buffer (pH 7) in acetonitrile at a flow rate of 1mL/min. Accuracy, precision and recovery of rabeprazole from plasma was determined. System suitability parameters such as theoritical plates, resolution, assymetry factor, LOQ and LOD are reported. The optimized method is simple, accurate, precise, robust and thereby could be used for the determination of bioeqvivalence of rabeprazole between drug products in human subjects.