Objective: The objective of the present investigation is to assess the biochemical analysis in the effluent sample collected from the nearby runoff of common effluent treatment plant (CETP) at Valayampet, Vaniyambadi, Tamilnadu, India, Methods: Biochemical characteristics of the effluent were determined as per the protocol derived by American public health association (APHA). Results: The results indicated that effluent sample was found toxic in nature, having high BOD, COD, TDS and Cr (VI) content (3.13 mg/L). Conclusion: As per the results obtained it was concluded that leather industry should make all attempts to reduce its impact on environment by making every effort to reuse and recycle chemical compounds.