Sivakumar S

Department of Social Sciences

School of Social Sciences & Languages

Vellore Campus

Santhoshkumar S

Vimal S

Abdul Majeed S

Taju G

Haribabu P

Uma A

Sahul Hameed A.S.

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Journal of Fish Diseases

Enterocytozoon hepatopenaei (EHP) is a microsporidian parasite that causes hepatopancreatic microsporidiosis (HPM) in penaeid shrimp. HPM was observed in several countries, including Thailand and India; it has become a prominent pathogen in shrimp culture. Based on observations on EHP infection in the wild, the route of transmission has been hypothesized. Identification of artificial EHP infection procedures can facilitate our understanding of EHP transmission. Experimental transmission of EHP was attempted using the immersion and oral infections of infection. In the immersion mode, post-larvae (PL) were exposed to an EHP tissue homogenate (0.2%) by immersion for 48 hr. Experimental samples were collected at various time points, and infection was confirmed using polymerase chain reaction, haematoxylin and eosin staining, transmission electron microscopy and modified trichrome staining. All test results revealed successful EHP transmission. Similar results were obtained through oral infection (oral infection). Innate immune gene expression patterns during infection were analysed; prophenoloxidase, crustin and superoxide dismutase were upregulated at 6, 6 and 48 hr post-challenge, respectively. Experimental infection procedures facilitate the development of diagnostic and prevention strategies. This is the first study demonstrating the experimental transmission of EHP in shrimp PL. © 2019 John Wiley & Sons Ltd

Experimental horizontal transmission of Enterocytozoon hepatopenaei in post-larvae of whiteleg shrimp, Litopenaeus vannamei

Pathogen virulence impacts have been increased in aquaculture and continuous observation was predominantly focused on Enterocytozoon hepatopenaei (EHP). Qualitative and Quantitative analysis was achieved by using Real time Loop mediated isothermal amplification (real-time LAMP) method. Optimization of amplification was attempted with three different temperatures (64, 66 and 68 °C) and accomplished with 68 °C. Sensitivity analysis was achieved in comparison with nested and real-time polymerase chain reaction to real-time LAMP. It has been proved to be without any cross reactivity with other aquatic pathogens like white spot syndrome virus, Infectious myonecrosis virus, Vibrio panaeicida and microsporidium seriolae. Real-time LAMP can be used in sensitive, specific and early detection of EHP in shrimp. This study was recorded as first attempt in detection and quantification of Enterocytozoon hepatopenaei in shrimp using real-time LAMP assay. © 2017 Elsevier B.V.

Aquaculture

Rapid and sensitive real-time loop meditated isothermal amplification for the detection of Enterocytozoon hepatopenaei of shrimp

Emergence of Enterocytozoon hepatopenaei (EHP) in farmed Penaeus (Litopenaeus) vannamei in India

Journal of Invertebrate Pathology

Development of in situ hybridization and PCR assays for the detection of Enterocytozoon hepatopenaei (EHP), a microsporidian parasite infecting penaeid shrimp

Trends in Parasitology

Microsporidia: diverse, dynamic, and emergent pathogens in aquatic systems

BMC Veterinary Research

The microsporidian Enterocytozoon hepatopenaei is not the cause of white feces syndrome in whiteleg shrimp Penaeus (Litopenaeus) vannamei

Enterocytozoon hepatopenaei sp. nov. (Microsporida: Enterocytozoonidae), a parasite of the black tiger shrimp Penaeus monodon (Decapoda: Penaeidae): Fine structure and phylogenetic relationships

Biochemical changes and tissue distribution ofEnterocytozoon hepatopenaei(EHP) in naturally and experimentally EHP-infected whiteleg shrimp,Litopenaeus vannamei(Boone, 1931), in India

Journal	Data powered by TypesetJournal of Fish Diseases
Publisher	Data powered by TypesetWiley
ISSN	0140-7775
Open Access	0