In this study, the effect of different modes of cultivation viz., photoautotrophic (AT), heterotrophic (HT) and photomixotrophic (MT), on the growth and biochemical responses of Chlorella sp. isolated from local ponds. The performance of microalgal growth was quantified using nonlinear growth models such as Gompertz, logistic, Baranyi-Roberts, Morgan under different cultivation conditions. The results revealed that microalgae could grow better in MT than in other cultivation modes with a major increase in biochemical responses for carbohydrates, which showed higher accumulation under HT. The maximum biomass concentration was 1.24 g L-1 (MT), 1.16 g L-1 (HT), 0.76 g L-1 (AT) with maximum specific growth rates of 0.0083 h-1 (HT), 0.0078 h-1 (MT) and 0.0055 h-1 (AT) respectively. The biomass concentration was higher in the order of MT>HT>AT for which MT yielded 8.8 fold higher biomass, compared with the initial biomass concentration, at the end of experiments (16 days). Concomitant increases in biochemical responses were observed in the three cultivation conditions. Protein and lipid accumulation in the MT mode was higher (1.2 fold) compared with the initial protein yield as well as the other cultivation modes. However, the carbohydrate yield was higher (1.12 fold) in the heterotrophic mode than in other cultivation conditions. © 2018 Assoc. Brasiliera de Eng. Quimica / Braz. Soc. Chem. Eng.. All rights reserved.