This study reports the development of a fast and facile route for the synthesis of novel TiO2-CeO2 nanocomposite film using sol-gel spin coating method with the mixture of commercial CeO2, titanium-iso-propoxide (TTIP) and aqueous ammonia. Solutions of TTIP in ethanol were mixed with CeO2 nanoparticles to form a mixed sol-gel. The sol containing TiO2-CeO2 was coated over the glass substrate and the resulting nanocomposite thin film were characterized in terms of phase and surface morphology by XRD, TEM, SEM and FT-IR. Conventional methods of the synthesis of CeO2–TiO2 nanocomposite require a long time, and TiO2 is rarely found as a coated material. In contrast, the sol-gel spin coating method was able to synthesize CeO2–TiO2 sol within a very short time. The particle size of the TiO2–CeO2 nano-composite film was found to be 23 nm. The Uric acid biosensor was fabricated by immobilizing mixed enzyme [urease (Urs) and glutamate dehydrogenase (GLDH)] on this nano-composite film showed a response time of 10 s, sensitivity as 0.9016 µAcm−2 mM−1 and detection limit of 0.165 mM. The value of Michaelis–Menten constant (Km) estimated using Line weaver–Burke plot as 4.8 mM which indicates the high affinity of uricase towards its analyte (uric acid). The enhanced sensitivity and linear range of the nanobiocomposite film obtained by cyclic voltammetry measurements suggested that the mixed enzyme and TiO2-CeO2 composite material has novel characteristics for uric acid determination in the human blood for the application of arthritis diseases. © 2019 Ain Shams University