The present study was focused on the biodegradation of monocrotophos (MCP) by indigenous bacteria isolated from sugarcane cultivable soil. The isolates were tested for pesticide tolerance, and the strain VITNNDJ5 showed maximum MCP tolerance and highest degradation potential. The presence of candidate gene for pesticide degradation and plant growth promoting rhizobacterial traits confirmed VITNNDJ5 to be the effective strain. Biodegradation of MCP (1000 mg L−1) was monitored using UV spectrophotometer and HPLC; the degradation products were identified by GCMS. The degradation kinetics was analyzed, and the rate constant (k) and half-life (t1/2) were calculated. Maximum degradation of up to 93% was observed within 5 days. The 16S rRNA gene sequencing revealed VITNNDJ5 to be the closest neighbor of Bacillus aryabhattai (GenBank accession number—KU598848). The augmentation of VITNNDJ5 to the rhizosphere of Liriope muscari enhanced the plant growth and degradation of MCP in soil. The fate of MCP in soil was analyzed, and a metabolic pathway was proposed. © 2019, Islamic Azad University (IAU).

Jabez Osborne W

Department of Bio-Sciences

School of Bio Sciences and Technology

Vellore Campus

Dash D.M

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

International Journal of Environmental Science and Technology

The bacterium Serratia marsescens strain JAS16 was isolated from agricultural soil which had prior exposure to monocrotophos for three years. The strain JAS16 tolerated up to 1200 mg L–1 monocrotophos and degraded the insecticide (1000 mg L–1) at a degradation rate constant of 136 d−1 (DT50 = 3.7 d). In soil, the degradation rate constant was 105 d−1 (DT50 = 4.8 d). A schematic pathway is being proposed from the degraded products derived from gas chromatography--mass spectrometry (GC-MS). The phytotoxicity of degradation products to Vigna radiata, Vigna unguiculata, and Macrotyloma uniflorum and the genotoxicity to Allium cepa roots were found to be low. A cost-effective powder-based formulation was achieved with the isolate. The isolate remained viable during the storage and also multiplied with a higher colony forming units (CFU) load g–1 for over a period of seven weeks of storage. © 2015 Taylor &amp; Francis.

Toxicological & Environmental Chemistry

Bacterial degradation of monocrotophos and phyto- and cyto-toxicological evaluation of metabolites

Several researchers have focussed on degradation of pesticides by microorganisms, which is the most viable option in remediation of agricultural soil. However, very few have illustrated the degradation of mixed pesticides. Hence, in the present investigation biodegradation of a mixture of both organophosphorus and organochlorine pesticide directed by microbial consortium was carried out in a fermentor-bioreactor system. The pesticides chosen were chlorpyrifos, monocrotophos (organophosphorus) and endosulfan (organochlorine). In order to accomplish an effective degradation, the microorganisms were isolated from agricultural fields which were previously exposed to the pesticides mentioned above. The bacteria were isolated by minimal meda with the individual pesticide as the only carbon source. The individual strains were identified by morphological, biochemical and 16S rRNA characterization. The bacterial consortium comprised of 10 organisms which were as follows: Alcaligenes sp. JAS1, Ochrobactrum sp. JAS2, Sphingobacterium sp. JAS3 which were screened and selected from chlorpyrifos contaminated soil, Enterobacter ludwigii JAS17, Pseudomonas moraviensis JAS18 and Serratia marcescens JAS16 which were isolated from monocrotophos spiked soil and Klebsiella pneumoniae JAS8, Enterobacter cloacae JAS7, Halophilic bacteria JAS4, Enterobacter asburiae strain JAS5 were obtained from endosulfan persistent soil sample. The fermentor-bioreactor system containing 2L of sterile minimal medium supplemented with 300mgl-1 of chlorpyrifos, 1000mgl-1 of monocrotophos, 1000mgl-1 of endosulfan as the only source of carbon and inoculated with 20ml of bacterial consortium (approximately 3×106cellsml-1). The HPLC and GC-MS studies were performed to record the degradation of mixed pesticides. © 2014 Taiwan Institute of Chemical Engineers.

Journal of the Taiwan Institute of Chemical Engineers

Simultaneous degradation of organophosphorus and organochlorine pesticides by bacterial consortium

Science of The Total Environment

A review on removal of organophosphorus pesticides in constructed wetland: Performance, mechanism and influencing factors

Water Research

Insight into a highly efficient electrolysis-ozone process for N,N-dimethylacetamide degradation: Quantitative analysis of the role of catalytic ozonation, fenton-like and peroxone reactions

African Journal of Microbiology Research

GC-MS and molecular analyses of monocrotophos biodegradation by selected bacterial isolates

Biodegradation of monocrotophos by a plant growth promoting Bacillus aryabhattai (VITNNDJ5) strain in artificially contaminated soil

Journal	Data powered by TypesetInternational Journal of Environmental Science and Technology
Publisher	Data powered by TypesetSpringer Science and Business Media LLC
ISSN	1735-1472
Open Access	0