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Biodegradation of perylene and benzo [ghi] perylene (5-6 rings) using yeast consortium : Kinetic study, enzyme analysis and degradation pathway
Mandal S.K,
Published in Triveni Enterprises
2018
Volume: 39
   
Issue: 1
Pages: 5 - 15
Abstract
Aim: The present work aims to demonstrate the ability of yeast consortium for degradation of perylene and benzo[ghi]perylene which may contribute towards the development of an appropriate in-situ bioremediation techniques. Methodology: Degradation of perylene (five ring) and benzo[ghi]perylene (six ring) was evaluated using yeast consortia under optimized conditions. Rate of biodegradation was tested with various kinetic models and intermediate metabolites were identified by GC-MS and FTIR analysis. Involvement of enzymes viz., 1,2-dioxygenase, 2,3-dioxygenase, catalase, laccase, lignin peroxidase and manganese peroxide were noted during PAHs degradation. Results: The yeast consortium YC02 (Hanseniaspora opuntiae NS02, Debaryomyces hansenii NS03 and Hanseniaspora valbyensis NS04)degraded67 {\%} perylene at initial concentration 50 mg l-1, whereas YC04 (Rhodotorula sp. NS01, Debaryomyces hansenii NS03 and Hanseniaspora valbyensis NS04) degraded 60 {\%} benzo[ghi]perylene at initial concentration 40mgl-1 after 6 days. The consortium YC02 and YC04 exhibited best reaction by first order kinetics with half life period of 3.397 days and 4.218 days for degradation of perylene and benzo[ghi]perylene, respectively. Interpretation: Based on the metabolites identified by GC-MS and FTIR analysis, possible pathways of perylene and benzo[ghi]perylene degradation were proposed. The results of present study revealed that application of yeast consortium can be a valuable approach towards remediation of high molecular weight PAHs having more benzenerings.
About the journal
JournalJournal of Environmental Biology
PublisherTriveni Enterprises
ISSN0254-8704
Open Access0