Present study demonstrates the decolourization and degradation of reactive green dye (RGD) by indigenous fungal strain VITAF-1 isolated from dye contaminated sites of Tirupur district, T.N, India. The intensive metabolic activity of VITAF-1 led to 97.9% decolourization of RGD within 48 h at 30 ± 2 °C temperature in Minimal Salt Media (MSM). VITAF-1 had a strong capability to decolourize elevated concentrations of reactive green dye (250 mg L−1–1500 mg L−1) over a pH range of 3.0–9.0. Induction of oxidoreductive enzymes like laccase, LiP and DCIP reductase confirmed their potential role in degradation. Decolourization assay with living and dead biomass of VITAF-1 revealed its ability in absorbing RGD. The degradation capability of the isolate VITAF-1 was monitored using UV–Vis spectrophotometer, HPLC and FTIR analysis, further the degraded product was identified as benzoic acid, 2(-1-oxopropyl) by GC-MS. 18S rRNA gene sequencing confirmed the fungal strain VITAF-1 to be the nearest neighbour of Aspergillus sp. © 2016 Elsevier Ltd

Jabez Osborne W

Department of Bio-Sciences

School of Bio Sciences and Technology

Vellore Campus

Sinha A

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

International Biodeterioration & Biodegradation

Contamination of soil by textile effluent is a major threat found worldwide. These pollutants have diverse range of negative effects on the ecosystem, therefore restoration through cost effective biological strategy is the need of the hour. The aim of the current study was to enhance the decolourization of reactive green dye (RGD) using phytoremediation coupled with augmentation of effective bacteria to the rhizosphere. The isolate Klebsiella sp. VITAJ23 was isolated from textile effluent polluted soil and was assessed for its plant growth promoting traits (PGP) and the PGP functional genes were amplified. The soil was artificially polluted with RGD concentration ranging from 1000 to 3000 mg kg −1 and Alternanthera philoxeroides plantlets were planted in phyto and rhizoremediation treatments, the setup was maintained upto 60 d. The isolate VITAJ23 was augmented in the rhizoremediation setup and the morphological parameters were assessed at regular interval. There was a significant increase in the chlorophyll content as well as root and shoot length of the plant when treated with the bacterial suspension. Decolourization study revealed 79% removal of reactive green dye with an enhanced oxido-reductase enzyme activity in the setup bioaugmented with bacteria. The biodegraded metabolites were identified as 2-allylnapthalene, l-alanine, n-acetyl-and propenoic acid by GC–MS analysis and a plant-bacteria degradation pathway was predicted using computational tools. Inoculation of PGP-Klebsiella sp. VITAJ23 enhanced the rate of plant growth and dye degradation. © 2018

Fulltext

Microbiological Research

Reactive green dye remediation by Alternanthera philoxeroides in association with plant growth promoting Klebsiella sp. VITAJ23: A pot culture study

Pollution by the release of dye containing wastewater has been alarming worldwide. Biodegradation of reactive green dye and textile effluent by Candida sp. VITJASS was investigated in dynamic condition. Paddy rhizosphere yeast Candida sp. VITJASS showed 84% removal of reactive green dye and 75% reduction in ADMI of textile effluent with a considerable synthesis of oxido-reductase enzymes. The degraded products were analyzed by UV-vis spectroscopy, HPLC and FTIR, which indicated the biotransformation of dye into non-toxic metabolites. GC-MS analysis revealed the formation of Diphenylmethane as potential end product. Phytotoxicity assessment on Phaseolus mungo seeds suggested the non- toxic nature of extracted metabolite. Toxicity studies on zebrafish revealed disturbed cellular integrity and disruption of tissues with crude dye supplementation. However, the degraded metabolites by VITJASS had an intact lumen morphology in the fishes. Thus, the present study depicts the augmentation of yeast strain to be efficacious and promising for degradation of dye with the synthesis of non-toxic metabolites. © 2018 Elsevier Ltd.

Journal of Environmental Chemical Engineering

Degradation of reactive green dye and textile effluent by Candida sp. VITJASS isolated from wetland paddy rhizosphere soil

Bioprocess and Biosystems Engineering

Microbial degradation, spectral analysis and toxicological assessment of malachite green by Streptomyces chrestomyceticus S20

Thermophilic Fungi

Taxonomy and Molecular Phylogeny of Thermophilic Fungi

Enzymatic decolorization and degradation of azo dyes – A review

Fungal Genetics and Biology

Wood and humus decay strategies by white-rot basidiomycetes correlate with two different dye decolorization and enzyme secretion patterns on agar plates

Azo and anthraquinone dye mixture decolourization at elevated temperature and concentration by a newly isolated thermophilic fungus, Thermomucor indicae-seudaticae

Iranian Journal of Environmental Health Science & Engineering

Decolorization and detoxification of Synozol red HF-6BN azo dye, by Aspergillus niger and Nigrosporasp

Biodegradation of reactive green dye (RGD) by indigenous fungal strain VITAF-1

Journal	Data powered by TypesetInternational Biodeterioration & Biodegradation
Publisher	Data powered by TypesetElsevier BV
ISSN	0964-8305
Open Access	0