In this work the bioreduction of Cr(VI) from aqueous solutions by Halomonas sp. was reported. Medium pH had significant effect on the bioreduction of Cr(VI). Glucose at 1000mgL-1 was essential for growth of Halomonas sp. as well as bioreduction of Cr(VI). A maximum specific Cr(VI) reduction rate of 0.74mgg-1h-1 was obtained for an initial Cr(VI) concentration of 40mgL-1. A maximum of 98.6% reduction of Cr(VI) was reported for an initial concentration of 10mgL-1. Other initial Cr(VI) concentrations studied viz. 20, 30 and 40mgL-1 resulted in 96.3%, 94.4% and 93.3% of Cr(VI) reduction respectively. pH 7 was found to be optimum for the bioreduction of Cr(VI) with free cells of Halomonas sp. Cell free extracts reported a maximum reduction efficiency of 89.65% for an initial metal concentration of 40mgL-1. The optimum pH for CFE on Cr(VI) reduction was 6.5. NADPH and NADH were found to be the best electron donors. EDTA, NaN3, KCN, PCP inhibited the reduction of Cr(VI) by cell free extract. © 2012 Elsevier B.V.