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Bioremediation of caffeine-contaminated soil by immobilized yeast - A laboratory based study
V. Lakshmi,
Published in
2012
Volume: 11
   
Issue: 3
Pages: 385 - 392
Abstract
The aim of this work was to ascertain the efficacy of immobilised yeast for the remediation of caffeine in soil under laboratory condition. Biostimulation with inorganic nutrients and bioaugmentation with caffeine utilizing single and mixed yeast cultures viz., Mixed culture 1, Mixed culture 2, Mixed culture 3 and Mixed culture 4 were employed as remedial options for the removal of caffeine from contaminated soil. To promote caffeine removal, biowaste materials (wheat bran, sawdust, peanut hull powder) were used as biocarriers for immobilization of caffeine degrading yeast species following the method of physical adsorption. We constructed laboratory biopiles to compare the treatment bioaugmentation with bioaugmentation combined biostimulation using single and mixed cultures of immobilized yeast. Maximum removal of caffeine was found to be 98.3 {\%} after 24 days in the biostimulated soil which was bioaugmented with sawdust immobilized yeast mixed culture No. 4 consisting of three yeast species viz., Trichosporon asahii, Candida tropicalis and Candida inconspicua. Dehydrogenase activity in the soil was remarkably enhanced to 639 $\mu$g TPF g-1 soil and microbial numbers were also increased for the soil treated under the same conditions described above. Phytotoxicity assay confirmed the reduction of caffeine toxicity in the contaminated soil after treatment. Thus, sawdust immobilized mixed yeast culture No. 4 could serve as potential tool for the remediation of caffeine from contaminated soil.
About the journal
JournalNature Environment and Pollution Technology
ISSN23953454