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Bioremediation of chromium (VI) by Bacillus aerophilus VIT SN strain
N.S. Pattanayak, R. Roy, M. Sathiavelu,
Published in Research Journal of Pharmaceutical, Biological and Chemical Sciences
2014
Volume: 5
   
Issue: 3
Pages: 533 - 545
Abstract
Bacterial strain (CDB1) which is more efficient to transform/reduce Cr (VI) to Cr (III) was isolated from Common Effluent Treatment Plant of Ranipet, Tamil Nadu. Based on biochemical characterization, it was identified as Bacillus sp. and after sequencing, it showed 99% similarities with Bacillus aerophilus and thus named as Bacillus aerophilus VIT SN strain. Optimum pH and temperature for the growth of bacterial isolate were found to be 7.5 and 37°C respectively. The isolated bacteria showed maximum growth on media containing 2% NaCl and they were able to survive in media containing K2Cr2O7 as source of Cr (VI) upto 500 ppm concentration. Although slight decrease of growth was also noticed when it was incubated in LB broth containing 100 ppm K2Cr2 O7 in comparison to control (absence of K2Cr2O7). The ability to reduce hexavalent chromium to trivalent form by Bacillus aerophilus VIT SN strain was estimated by using Atomic Absorption Spectroscopy (AAS). It showed 95%, 94%, 90%, 85% and 73% degradation ability for 100 ppm, 200ppm, 300ppm, 400ppm and 500ppm concentration of K2Cr2O7respectively as source of Cr (VI). The bacterial strain showed maximum growth in culture broth containing Dextrose (DEX) and Yeast Extract (YE) supplemented as carbon and nitrogen sources respectively. Apart from Chromium, Bacillus aerophilus VIT SN strain also showed resistance to other heavy metals such as Cadmium (500ppm), Lead (500ppm), Zinc (500ppm) and Copper (300ppm) up to certain concentration.
About the journal
JournalResearch Journal of Pharmaceutical, Biological and Chemical Sciences
PublisherResearch Journal of Pharmaceutical, Biological and Chemical Sciences
ISSN09758585