Endosulfan is Cyclodiene organo chlorine currently used as an insecticide all over the world and its residues are posing a serious environmental threat causing long-term harm to human and wildlife. In the present study endosulfan degrading 4 bacterial strains (B1: Staphylococcus B2: Micrococcus B3: Bacillus B4: Pseudomonas) and 5 fungal strains (F1: Mucor F2: Pencillium F3: Aspergillus fumigates F4: Candida F5: Yeast) were isolated from cashew growing soil of Kasargode district at Kerala. The isolated strains were determined for endosulfan degradation potential in lab scale and in pilot plant study. In the lab scale, all the 9 strains differed substantially in their potential to degrade endosulfan ranging from 88 to 93% of the spiked amount (100 mg l-1). The degradation efficiency of the microbes was confirmed in the pilot plant studies with bacterial and fungal consortium. The initial concentration of endosulfan in pilot plant studies was 1000 mg/kg. Bacterial consortium showed 82.5% of degradation after 30 days and fungal consortium showed 80.3% of degradation. B2, F2, bacterial consortium and fungal consortium were analyzed using GC-MS and the degraded product was found to be endosulfan lactone or endolactone. Thus the result of the present study implies that microbial degradation of endosulfan by soil microbes may provide a basis for the development of bioremediation strategies to remediate the pollutants in the environment.