An increasing number of hazardous heavy metals are discharged into the environment through natural and anthropogenic activity, creating serious environmental problems. The toxicity of chromium and copper affects soil and water, which further harms microbes, animals and plants. Biosorption has proved to be an effective method in expulsion of heavy metals from environment. Accumulation of heavy metals by algae has been observed by earlier works. In this study, Spirogyrahas been examined for biosorption capacity of copper and chromium in different conditions. Various parameters, such as, initial metal concentrations; biomass and pH were considered for the experiment. The biosorption capacity was checked by flame atomic absorption spectrophotometer. The optimum metal concentrations were 10 mg/L for chromium and 20 mg/L for copper. 2.5 g/L biomass was observed to show the maximum biosorption at pH 6. The contact time throughout the experiment was maintained for 1 h. © 2015, Sphinx Knowledge House. All Rights Reserved.